BioProScale Symposium "Inhomogenieties in large-scale bioreactors: Description – scaling – control"

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Wednesday, 14 March 2012
13:00 Welcome address
Peter Neubauer (Department of Bioprocess Engineering, TU Berlin, Germany)
13:15 Opening Lecture
Lifelines of single cells and populations in large scale bioreactors – Complex dynamic interplay between extracellular environment and cell machinery (L01) 
Matthias Reuss (University of Stuttgart, Germany)
Chair: Henk Noorman (DSM), Stefan Junne (TU Berlin)
14:00 Physiological heterogeneity and population dynamics in microbial cultures (L02) 
Chris Hewitt (Loughborough University, United Kingdom)
14:40 Coffee break with poster session and exhibition 
15:10 Key note lecture: Population composition and dynamics in biogas reactors (L03)   
Edith Nettmann, Antje Rademacher, Kathrin Heeg, Michael Klocke (Leibniz-Institut für Agrartechnik Potsdam-Bornim e.V. (ATB), Germany)
15:40 Reduced overflow of acetate in Escherichia coli by gene engineering and media design (L04)
Kaarel Adamberg, Kaspar Valgepea, Ranno Nahku, Petri-Jaan Lahtvee, Liisa Arike, Sten Erm, Raivo Vilu (Tallinn University of Technology and CCFFT, Estonia)
16:05 Tuning protein production at level of translation (L05)
Zoya Ignatova (University of Potsdam, Germany)
16:30 Temperature and substrate-related stresses enhance recombinant protein production (L06) 
Mohammedhadi Jazini, Christoph Herwig (Vienna University of Technology, Austria)
16:55 Coffee break with poster session and exhibition 
17:10 Synthesis of non-proteinogenic amino acid species in recombinant Escherichia coli  fermentation – Looking for strain dependent  differences (L07)     
Michael Biermann, Uwe Horn (Hans-Knöll Institut, Jena, Germany), Guido Seidel (Wacker Biotechnologie, Jena, Germany)    
17:35 Effects of oxygen transfer limitation and inhomogeneous supply in Corynebacterium glutamicum (L08)
Friedrich Käss, Marco Oldiges (Forschungszentrum Jülich, Germany)
18:00 Effect of intensity and frequency of glucose pulse perturbations on transient E. coli behavior: A step toward the large scale bioreactor (L09)
Sirichai Sunya, Jean-Louis Uribelarrea, Carole Molina-Jouve, Nathalie Gorret (Université de Toulouse, INRA, CNRS, France), Frank Delvigne (University of Liege, Belgium)
18:25 The dynamics of the branched chain amino acid synthesis in Escherichia coli in a two-compartment reactor (L10)
Stefan Junne, Eva Brand, Dennis Runge, Martin Baudis, Peter Neubauer (TU Berlin, Germany)
18:50 Distinguished lecture: Engineering of the genetic code as a biosafety strategy in the industrially relevant bio-production? (L11)
Nediljko Budisa (TU Berlin, Germany)
19:30 Poster session, Exhibition and Welcome Reception
21:30 End 
Thursday, 15 March 2012
Chair: Matthias Reuss (University of Stuttgart), Peter Neubauer (TU Berlin)
9:00 Key note lecture: Bioprocess monitoring by marker gene analysis (L12)
Thomas Schweder (Ernst-Moritz-Arndt Universität Greifswald, Germany)
9:40 Elaboration of a mini scale-down platform on the basis of the response of GFP microbial biosensors responsive to a substrate limitation: Detection of substrate heterogeneities at the single cell level and assessment of microbial viability (L13)
Alison Brognaux, Philippe Thonart, Frank Delvigne (Université de Liège, Belgium), Peter Neubauer (TU Berlin), Jean-Claude Twizere
10:05 Microthrix parvicella and Cloacamonas acidaminovorans: Indicator organisms for foam formation in large-scale biogas plants? (L14)
Tobias Lienen (Helmholtz-Zentrum Potsdam Deutsches GeoForschungsZentrum GFZ, Germany)
10:30 Evaluation of evolved xylose fermenting strains for bioethanol production - Comparison of single cells and mixed populations  (L15)
Elia Tomas-Pejo, Lisbeth Olsson (Chalmers University of Technology, Sweden)
10:55 Coffee break and poster session and exhibition 
11:25 Systematic process development from microscale to pilot production at the example of a production of heterologeous ribonuclease inhibitor in E. coli (L16)
Juozas Siurkus (Thermo Fisher Scientific (formerly Fermentas), Lithuania), Peter Neubauer (TU Berlin, Germany)
11:50 Engineering E. coli to increase plasmid DNA production in high cell-density cultivations in batch mode (L17)
Alvaro R. Lara (Universidad Autónoma Metropolitana-Cuajimalpa, México)
12:30 Lunch break, poster session and exhibition
Bioreactor environment
Chair: Octavio Ramirez (Instituto de Biotecnologica, UNAM), Thomas Schweder (Ernst-Moritz-Arndt Universität Greifswald)
14:00 Key note lecture: Scale up of lactic acid production (L18)
Joachim Venus (Leibniz-Institut für Agrartechnik Potsdam-Bornim e.V. (ATB), Germany)
14:40 Generic methods for scale up of bioprocesses based on quality-by-design (QbD) principles (L19)
Christoph Herwig (Vienna University of Technology, Austria)
15:15 Shaken bioreactors with culture volumes of 0.1 ml to 200 liter: Effects of scale, vessel geometry and shaking parameters on kLa values, mixing times and hydrodynamics (L20)
Wouter Duetz (Enzyscreen B.V., The Netherlands), Tibor Anderlei, Markus Kühner (Kühner AG, Switzerland)
15:50 Physiological impacts of mixing at the docosahexaenoic acid production process with the heterotrophic marine microalgae Crypthecodinium cohnii (L21)
Friederike Hillig, Stefan Jahns, Stefan Junne, Peter Neubauer (TU Berlin, Germany)
16:15 Coffee break and poster session and exhibition
16:30 Design of a scalable single-use bioreactor (L22)
Nico M.G. Oosterhuis, Anton Tromper (Cellution Biotech B.V., The Netherlands), Stefan Junne,  Peter Neubauer (TU Berlin, Germany)
16:55 Towards a complete single-use upstream process for an Escherichia coli high cell density fermentations (L23)
Thomas Dreher, C. Zahnow, U. Husemann, G.Greller (Sartorius-Stedim, Germany)
17:20 Decreasing the uncertainty when increasing the scale (L24)
Wouter van Winden, Rogier Meulenberg, Sybe Hartmans (DSM Biotechnology Center, The Netherands)
17:50 Insitu Biocell Vitality Analyzer: The on-line bridge to flow cytometry (L25)
Friedel H. Schwartz (Sequip S + E GmbH, Germany)
18:10 EnBase: A solution for fed-batch conditions during process development (L26)
Antje Neubauer (BioSilta Europe GmbH, Germany)
19:30 Conference dinner
Friday, 16 March 2012
Bioreactor environment
Chair: Nico Oosterhuis (Cellution Biotech), Kathrin Ralla (TU Berlin)
9:00 Key note lecture: Consistent bioprocess development and scaling  (L27)    
Octavio T. Ramirez (Universidad Nacional Autónoma de México, México) 
9:40 Scale-down of penicillin production in Penicillium chrysogenum (L28)
Walter van Gulik (Delft University of Technology, The Netherlands)
10:10 Development of a scale-down model of hydrodynamic stress to study the performance of an industrial CHO cell line under simulated production scale bioreactor conditions (L29)
Jochen Sieck (Novartis Pharma AG, Switzerland)
10:35 Scale-down model of the inactivated Polio Vaccine production process: Unit operation cell and virus culture (L30)
Yvonne E. Thomassen, Leo A. van der Pol, Wilfried A.M. Bakker (RIVM, The Netherlands)
11:00 Coffee break with poster session and exhibition
11:30 Are classical correlations suitable for high performance lab scale bioreactors? (L31)
Sebastian Schaepe, Andreas Lübbert (Martin-Luther-Universität Halle-Wittenberg, Germany), Artur Kuprijanov, Rimvydas Simutis (Kaunas Technical University, Lithuania)
11:55 Evaluation of process integration opportunities using process simulation and scheduling tools (L32)
Jose Oscar Jimenez (Intelligen B.V., The Netherlands)
12:20 Lunch break with poster session and exhibition
13:30 Bacteriophage contamination in bacterial fermentation as an important cause of fermentation failures (L33)
Marcin Los, Piotr Golec, Joanna M. Los, Grzegorz Wegrzyn (University of Gdansk, Poland)
13:55 High throughput cultivation of recombinant Escherichia coli in microtiter plates (L34)
Csilla Török (Austrian Centre of Industrial Biotechnology, Vienna), Monika Cserjan, Gerald Striedner (Austrian Centre of Industrial Biotechnology / University of Natural Resources and Applied Life Sciences, Vienna)
14:20 Picoliter bioreactors for growth heterogeneity studies of industrial bacteria on single cell level (L35) 
Alexander Grünberger (FZ Jülich, IBG-1: Biotechnologie, Germany)
14:45 Concluding remarks      
Peter Neubauer (TU Berlin, Germany)
15:00 End of symposium
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